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Service Information and Rates

 

Oligo Synthesis (Shipping and Handling)

Peptide Synthesis (Shipping and Handling)

Gene Expression

Mass Spectrometry

Real-Time PCR

Protein Analytics
   • Mass Mapping
   • Edman Sequencing

Pyrosequencing

Next Generation Sequencing

Surface Plasmon Resonance (Biacore)

DNA (Sanger) Sequencing & Fragment Analysis (GeneScan)

Single Cell Genomics

RNA/DNA QC
   • Agilent Bioanalyzer QC
   • AATI Fragment Analyzer QC


** We only accept PTA or PO# as a form of payment. We are currently not set up for any credit card transactions **
**Non-Profit and Commercial charges include administrative costs


GENOMIC SERVICES
Oligo Synthesis (top)
On Campus
Non-Profit
Commercial
• DNA (per base):
• 40nmole (standard scale)
• 200nmole
• 1µmole
• DNA bulk orders (per base):
• 40nmole (8-15 oligos/order)
• 40nmole (16-23 oligos/order)
• 40nmole or 25nmole (≥24 oligos/order)
• 25nmole - 96-well plate format by request (≥48 oligos/order)
• RNA (per base):
• 40nmole
• 200nmole
• Purification (per oligo):
• 40nmole and 200nmole synthesis scales
• 1umole scale
• Modification:
• Biotin -- (**For any orders with 3 or more modifications/order, please inquire.)
• TagMan Probe reporters and quenchers
• Inosine
• dU
• 5' Phosphorylation
• 5' Thiolation
• Internal mixed bases
• Phosphothiolation (antisense)
• Other modifications
• LNA (per base)
**Shipping and Handling :
• USA
• International
0.39
0.99
2.10

0.32
0.27
0.13
0.13
7.20
9.20
9.75
20

50
60
10
10

10

Inquire
no charge
no charge
Inquire
Inquire
no charge
51
0.50
1.29
2.73

0.42
0.35
0.17
0.17
9.36
11.96
12.70
26

65
78
13
13
13
Inquire
no charge
no charge
Inquire
Inquire
29
51
0.70
1.79
3.78

0.58
0.49
0.23
0.23
12.96
16.56
17.50
36

90
108
18
18
18
Inquire
no charge
no charge
Inquire
Inquire
29
51
Gene Expression (top)
On Campus
Non-Profit
Commercial
Expression Analysis: Full Services (** Chip is not included **)  
3' Array:
-- cDNA synthesis to data acquisition; includes Bioanalyzer QC
.
Standard (>50ng): using Affymetrix 3' IVT Plus protocol.
Small (500pg - 50ng): using Affymetrix 3' IVT Pico protocol
Gene Array:
-- Whole Transcript (WT) Sense Target Labeling to data acquisition.
Standard (>50ng): using Affymetrix WT Plus protocol.
Small (500pg - 2ng): using Affymetrix WT Pico protocol.
Human Transcriptome Array 2.0 (HTA 2.0)/Mouse Transcriptome Array 1.0 (MTA 1.0)/Rat Transcriptome Array 1.0 (RTA 1.0)
-- Interrogates exons for coding transcripts, exon-exon splice junctions and non-coding transcripts.
miRNA Array:
-- miRNA coverage of 71 organisms, including human, mouse, rat, canine, and rhesus macaque
.


470
495
455
460

525
210


610
645
590
595

680
275


845
890
820
828
945
378
Whole Genome DNA Analysis: Full Service (** Chip is not included **)  
• Cytoscan
-- Starting Material: genomic DNA
Inquire
Inquire
Inquire
Other Services  
Hybridization and Scanning (** Chip is not included **)
103
134
185
Real-Time PCR (top)
On Campus
Non-Profit
Commercial
• Instrument run: (per plate)
-- Client prepares plate ready to run
• Full Service: (per sample)
• Set-up fee (per order) -- Prices do not include primers
• Reverse Transcription -- (Bioanalyzer QC included)
• qPCR (triplicate) -- using either Taqman or SybrGreen Chemistry
• Custom Assay -- Taqman qPCR optimization
• Custom Assay -- SybrGreen qPCR optimization
28
Inquire
35
13
Inquire
Inquire
36
Inquire
46
17
Inquire
Inquire
50
Inquire
63
23
Inquire
Inquire
Pyrosequencing/PyroMark (top)
On Campus
Non-Profit
Commercial
New Project Consultation
PCR and Pyrosequencing Run/Sample (one target region)
• Run Only
-- PAN captures biotinylated product and runs client generated PCR products

Self Service: Instrument Run Only
-- Client prepares and runs plate; Reagents and consumables are not included but can be purchased from PAN.

Inquire
17
8.50
27
Inquire
22
12
35
Inquire
30
16
48
Next Generation Sequencing (top)
On Campus
Non-Profit
Commercial
Library Prep Services: (Price per Preparation including QC)
Low Input RNA Library Preparation:
• Clontech SMARTer Stranded Total RNA-Seq
• NuGen/Nextera
RNA Library Preparation:
• Illumina TruSeq Stranded Total RNA
• Illumina TruSeq Stranded mRNA
DNA Library Preparation:
• Nextera XT DNA
• MiSeq Services:
MiSeq Run Only:
--
Client provides MiSeq reagent kit with samples preloaded into the cartridge.
• 2 x 25 cycles
• 2 x 75 cycles
• 2 x 150 cycles
• 2 x 250 cycles
• 2 x 300 cycles
MiSeq Set-up and Run:
-- PAN provides MiSeq reagent kit and initial sample QC on Agilent 2100 Bioanalyzer.
• 2 x 25 cycles
• 2 x 75 cycles
• 2 x 150 cycles
• 2 x 250 cycles
• 2 x 300 cycles

275
350

245
225

185


180
291
365
515
625


878
1184
1258
1505
1615

357
455

318
293

240


231
373
467
659
800


929
1266
1360
1649
1790

495
630

440
405

330


335
541
679
958
1162


1033
1434
1572
1948
2152
DNA Sequencing & Fragment Analysis (top)
On Campus
Non-Profit
Commercial
• DNA Sequencing:
Per Sample Price:
Full Service
Intermediate Service (Clean-up & Run)
Economy Service (Run Only)
Per Plate Price:
• Full Service
• Run Only
• Fragment Analysis (GeneScan):
Clean-up and Run
Run only

7.75
5.50
4.40

580
208

7.75
5.50

10
7.75
6.50

755
270

10
7.75

13.95
9.90
7.90

950
375

13.60
9.80
Single Cell Genomics (top)
On Campus
Non-Profit
Commercial
Single Cell -- Instrument Run Only (Fluidigm C1)
-- Client provides Chip, C1 Single Cell Auto Prep Reagents, SMARTer Ultra Low RNA reagents and sample QC
Single Cell Capture and cDNA Amplification (96 cell chip) -- Full Service (** Chip is not included **)
-- PAN provides Single Cell Auto Prep Reagents, SMARTer Ultra Low RNA reagents and sample QC
NexteraXT cDNA Library Prep (per sample)
Single Cell Capture and cDNA Amplification (800 cell chip) -- Full Service (** Chip is not included **)
-- PAN provides Single Cell Auto Prep Reagents, SMARTer Ultra Low RNA reagents and sample QC
200
975
43
Inquire
260
1268
56
Inquire
360
1755
77
Inquire
RNA/DNA QC (top)
On Campus
Non-Profit
Commercial
Agilent Bioanalyzer QC (per sample)
• Nano RNA or DNA 1000 (up to 12 samples), or Pico RNA (up to 11 samples)
         ° each additional sample
• Small RNA or High-Sensitivity DNA (up to 11 samples)
         ° each additional sample
AATI Fragment Analyzer QC (per 96-well - plate format only)

17.50
14.50
22
17.50
350

23.50
19.25
28.50
24
455

31.50
26
39.50
33
600
PROTEIN SERVICES
Peptide Synthesis (top)
(Non-purified peptide synthesis includes analytical HPLC and Mass spec QC)
On Campus
Non-Profit
Commercial
• Stadard scale: (0.2 mmole-200 mg-500 mg)/amino acid (non purified)
• Set-up fee (includes first 5 Amino Acids)
• Per additional Amino Acid (>5 AA)
• HPLC Purification
• Modification
**Shipping and Handling :
• USA
• International
250
53
245
Inquire
no charge
51

325
69
318
Inquire
29
51

450
95
441
Inquire
29
51
Mass Spectrometry (top)
On Campus
Non-Profit
Commercial
• Mass spectrometry by MALDI: (per sample)
• Peptides (500-5000Da)
• Other (including proteins, DNA, polymer, small molecules)
• Prespotted plate
• Zip Tip Cleanup

18
31
12
14

24
40
15
19

34
56
22
25
Protein Analytics (top)
On Campus
Non-Profit
Commercial
Mass Mapping: (Protein Identification)
-- In-gel digestion with trypsin; MS/MS peptide fragmentation; Mascot search against the NCBInr protein database; Report summary in pdf format.
• Price per sample (for first 10 samples in an order)
• Additional sample(s) (above 10 samples in the order)
• Specialized database construction
• Protein ID/Characterization from complex protein mixtures
Edman Sequencing:
• Set-up fee/sample (includes first 7 Residues)
• Per residue (>7 residues)

159
103
Inquire
Inquire

210
29

208
136
Inquire
N/A

310
38

288
193
Inquire
N/A

410
53
Surface Plasmon Resonance -- Biacore (top)
(Experimental set-up includes generation of biosensor surfaces for ligand capturing: Direct or indirect capture)
On Campus
Non-Profit
Commercial
• Set-up fee (per chip, user provides chip)
• Experiment:
• Full-day duration
• Half-day duration
• User-operated instrument session (per hour)
• Biacore 3000
• T200
• User-operated instrument session (per day)
• Biacore 3000
• T200
• Consulting or data analysis (half-day duration)
250
445
215

64
78

355
425
227
350

620
300

96
125

460
550
317
525

890
430

128
163

640
765
435


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