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Service Information and Rates

Oligo Synthesis (Shipping and Handling) Peptide Synthesis (Shipping and Handling)
Gene Expression & Bioanalyzer QC Mass Spectrometry
Real-Time PCR Protein Analytics
   • Mass Mapping
   • Edman Sequencing
Pyrosequencing
Next Generation Sequencing Surface Plasmon Resonance (Biacore)
DNA Sequencing & Fragment Analysis Antibody

** We only accept PTA or PO# as a form of payment. We are currently not set up for any credit card transactions **


Oligo Synthesis (top)
On Campus
Non-Profit
Commercial
• DNA (per base):
• 40nmole (standard scale)
• 200nmole
• 1µmole
• DNA bulk orders (per base):
• 40nmole (8-15 oligos/order)
• 40nmole (16-23 oligos/order)
• 40nmole or 25nmole (>24 oligos/order)
• 25nmole - 96-well plate format by request (>48 oligos/order)
• RNA (per base):
• 40nmole
• 200nmole
• Purification (per oligo):
• 40nmole and 200nmole synthesis scales
• 1umole scale
• Modification:
• Biotin -- (**For any orders with 3 or more modifications/order, please inquire.)
• Inosine
• dU
• 5' Phosphorylation
• 5' Thiolation
• Internal mixed bases
• Phosphothiolation (antisense)
• Others
• LNA (per base)
**Shipping and Handling :
• USA
• International
0.37
0.95
1.95

0.30
0.25
0.10
0.10
6.75
8.50
8.95
17.95
50
10
10

10

Inquire
no charge
no charge
Inquire
Inquire
no charge
45
0.42
1.05
2.15

0.35
0.30
0.10
0.10
8.50
11
8.95
17.95
55
10
10
10
Inquire
no charge
no charge
Inquire
Inquire
25
45
1.15
1.80
3.90

0.80
0.50
0.10
0.10
10.50
12.75
8.95
17.95
80
10
10
15
Inquire
no charge
no charge
Inquire
Inquire
25
45
Gene Expression & Bioanalyzer (top)
On Campus
Non-Profit
Commercial
Expression Analysis: Full Services (** Chip is not included **)
3' Array:
-- cDNA synthesis to data acquisition; includes Bioanalyzer QC
.
Standard (>50ng): using Affymetrix 3' IVT Express protocol.
Small (500pg - 50ng): using NuGen protocol or PAN 2-cycle amplification protocol.
Gene Array:
-- Whole Transcript (WT) Sense Target Labeling to data acquisition.
Exon Array:
-- Survey of alternative splicing and gene expression - exon level analysis.
Tilling Array:
-- High-density tiled microarrays for transcript mapping and chromatin immunoprecipitation human, mouse, yeast
.
Only one array:
• Starting Material: DNA
• Starting Material: RNA
One Set (7 arrays):
• Starting Material: DNA
• Starting Material: RNA
miRNA Array:
-- miRNA coverage of 71 organisms, including human, mouse, rat, canine, and rhesus macaque
.


415
455
400
461

231
461

718
923
182


530
550
498
575

282
575

866
1108
228


775
775
698
825

400
825

1230
1563
343
Whole Genome DNA Analysis: Full Service (** Chip is not included **)
-- Starting Material: genomic DNA
• Human SNP Array 5.0 (1 chip)
• Human SNP Array 6.0 (1 chip)
• Cytoscan

410
441
Inquire

484
522
Inquire

615
665
Inquire
Other Services  
Hybridization and Scanning
Agilent Bioanalyzer QC (per sample)
• Nano or DNA 1000 (up to 12 samples), or Pico (up to 11 samples)
         ° each additional sample
• Small RNA or High-Sensitivity DNA (up to 11 samples)
         ° each additional sample
• Protein 230
87

14
11
16
14
Inquire
110

22
19
26
23
Inquire
156

28
23
32
27
Inquire
Real-Time PCR (top)
On Campus
Non-Profit
Commercial
• Instrument run: (per plate)
-- Client prepares plate ready to run
• Full Service: (per sample)
• Set-up fee (per order) -- Prices do not include primers
• Reverse Transcription -- (Bioanalyzer QC included)
• qPCR (triplicate) -- using either Taqman or SybrGreen Chemistry
• Custom Assay -- Taqman qPCR optimization
• Custom Assay -- SybrGreen qPCR optimization
24
Inquire
29
10
Inquire
Inquire
30
Inquire
40
15
Inquire
Inquire
37
Inquire
44
18
Inquire
Inquire
Pyrosequencing/PyroMark (top)
On Campus
Non-Profit
Commercial
New Project Consultation
PCR and Pyrosequencing Run/Sample (one target region)
Run Only
• Self Service: Instrument run
-- Client prepares samples ready to run
Inquire
13
6
Inquire
Inquire
16
10
Inquire
Inquire
25
12
Inquire
Next Generation Sequencing (top)
On Campus
Non-Profit
Commercial
Sample Prep Full Service
• MiSeq Services:
MiSeq Run Only:
--
Client provides MiSeq reagent kit with samples preloaded into the cartridge.
• 2 x 25 cycles
• 2 x 75 cycles
• 2 x 150 cycles
• 2 x 250 cycles
MiSeq Set-up and Run:
-- PAN provides MiSeq reagent kit and initial sample QC on Agilent 2100 Bioanalyzer.
• 2 x 25 cycles
• 2 x 75 cycles
• 2 x 150 cycles
• 2 x 250 cycles
Inquire


180
291
365
515


878
1184
1258
1505
Inquire


231
373
467
659


929
1266
1360
1649
Inquire


335
541
679
958


1033
1434
1572
1948
DNA Sequencing & Fragment Analysis (top)
On Campus
Non-Profit
Commercial
• DNA Sequencing:
Per Sample Price:
Full Service
Intermediate Service (Clean-up & Run)
Economy Service (Run Only)
Per Plate Price:
• Full Service
• Run Only
• Fragment Analysis (GeneScan):
Clean-up and Run
Run only

5
3
2

450
150

6
4

8
5
4

750
300

9
7

10
6
5

900
450

11
10.50
Peptide Synthesis (top)
(Non-purified peptide synthesis includes analytical HPLC and Mass spec QC)
On Campus
Non-Profit
Commercial
• Stadard scale: (0.2 mmole-200 mg-500 mg)/amino acid (non purified)
• Set-up fee (includes first 5 Amino Acids)
• Per additional Amino Acid (>5 AA)
• Immunogen scale: (25 µmole-10 mg-30 mg)/amino acid (non-purified)
• Set-up fee (includes first 5 Amino Acids)
• Per additional Amino Acid (>5 AA)
• HPLC Purification
• Modification
**Shipping and Handling :
• USA
• International
210
46
120
25
210
Inquire
no charge
45

249
49

156
33
242
Inquire
25
45

325
69

228
48
349
Inquire
25
45
Mass Spectrometry (top)
On Campus
Non-Profit
Commercial
• Mass spectrometry by MALDI: (per sample)
• Peptides (500-5000Da)
• Other (including proteins, DNA, polymer, small molecules)
• Prespotted plate
• Zip Tip Cleanup

15
25
10
11

20
34
12
11

26
45
18
11
Protein Analytics (top)
On Campus
Non-Profit
Commercial
Mass Mapping: (Protein Identification)
-- In-gel digestion with trypsin; MS/MS peptide fragmentation; Mascot search against the NCBInr protein database; Report summary in pdf format.
• Price per sample (for first 10 samples in an order)
• Additional sample(s) (above 10 samples in the order)
• Specialized database construction
• Protein ID/Characterization from complex protein mixtures
Edman Sequencing:
• Set-up fee/sample (includes first 7 Residues)
• Per residue (>7 residues)

148
96
Inquire
Inquire

218
24

192
125
Inquire
N/A

250
35

266
178
Inquire
N/A

360
48
Surface Plasmon Resonance -- Biacore (top)
(Experimental set-up includes generation of biosensor surfaces for ligand capturing: Direct or indirect capture)
On Campus
Non-Profit
Commercial
• Set-up fee (per chip, user provides chip)
• Experiment:
• Full-day duration
• Half-day duration
• User-operated instrument session (per hour)
• Biacore 3000
• T200
• User-operated instrument session (per day)
• Biacore 3000
• T200
• Consulting or data analysis (half-day duration)
200
340
170

50
63

285
340
175
305

520
260

77
103

350
410
255
425

698
349

102
130

506
615
336
Antibody (top)
On Campus
Non-Profit
Commercial
› All fees
Inquire
Inquire
Inquire


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