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Service Information and Rates

Oligo Synthesis (Shipping and Handling) Peptide Synthesis (Shipping and Handling)
Gene Expression & Bioanalyzer QC Mass Spectrometry
Real-Time PCR Protein Analytics
   • Mass Mapping
   • Edman Sequencing
Pyrosequencing
Next Generation Sequencing Surface Plasmon Resonance (Biacore)
DNA Sequencing & Fragment Analysis Antibody

** We only accept PTA or PO# as a form of payment. We are currently not set up for any credit card transactions **


Oligo Synthesis (top)
On Campus
Non-Profit
Commercial
• DNA (per base):
• 40nmole (standard scale)
• 200nmole
• 1µmole
• DNA bulk orders (per base):
• 40nmole (8-15 oligos/order)
• 40nmole (16-23 oligos/order)
• 40nmole or 25nmole (>24 oligos/order)
• 25nmole - 96-well plate format by request (>48 oligos/order)
• RNA (per base):
• 40nmole
• 200nmole
• Purification (per oligo):
• 40nmole and 200nmole synthesis scales
• 1umole scale
• Modification:
• Biotin -- (**For any orders with 3 or more modifications/order, please inquire.)
• Inosine
• dU
• 5' Phosphorylation
• 5' Thiolation
• Internal mixed bases
• Phosphothiolation (antisense)
• Others
• LNA (per base)
**Shipping and Handling :
• USA
• International
0.38
0.95
2

0.31
0.26
0.11
0.11
6.95
8.75
9.20
18.50
50
10
10

10

Inquire
no charge
no charge
Inquire
Inquire
no charge
47
0.43
1.08
2.20

0.36
0.31
0.12
0.12
8.75
12
9.20
18.50
55
10
10
10
Inquire
no charge
no charge
Inquire
Inquire
26
47
1.19
1.85
4

0.82
0.52
0.15
0.15
10.80
13
9.20
18.50
80
10
10
15
Inquire
no charge
no charge
Inquire
Inquire
26
47
Gene Expression & Bioanalyzer (top)
On Campus
Non-Profit
Commercial
Expression Analysis: Full Services (** Chip is not included **)
3' Array:
-- cDNA synthesis to data acquisition; includes Bioanalyzer QC
.
Standard (>50ng): using Affymetrix 3' IVT Express protocol.
Small (500pg - 50ng): using NuGen protocol or PAN 2-cycle amplification protocol.
Gene Array:
-- Whole Transcript (WT) Sense Target Labeling to data acquisition.
Exon Array:
-- Survey of alternative splicing and gene expression - exon level analysis.
Human Transcriptome Array 2.0 (HTA 2.0)/Mouse Transcriptome Array 1.0 (MTA 1.0)
-- Interrogates exons for coding transcripts, exon-exon splice junctions and non-coding transcripts.
miRNA Array:
-- miRNA coverage of 71 organisms, including human, mouse, rat, canine, and rhesus macaque
.


425
470
410
475
475
195


545
567
512
592
592
245


799
799
719
850
850
367
Whole Genome DNA Analysis: Full Service (** Chip is not included **)
-- Starting Material: genomic DNA
• Human SNP Array 5.0 (1 chip)
• Human SNP Array 6.0 (1 chip)
• Cytoscan

420
454
Inquire

498
537
Inquire

633
685
Inquire
Other Services  
Hybridization and Scanning
Agilent Bioanalyzer QC (per sample)
• Nano RNA or DNA 1000 (up to 12 samples), or Pico RNA (up to 11 samples)
         ° each additional sample
• Small RNA or High-Sensitivity DNA (up to 11 samples)
         ° each additional sample
90

15
12
18
16
114

23
20
29
26
161

30
24
36
30
Real-Time PCR (top)
On Campus
Non-Profit
Commercial
• Instrument run: (per plate)
-- Client prepares plate ready to run
• Full Service: (per sample)
• Set-up fee (per order) -- Prices do not include primers
• Reverse Transcription -- (Bioanalyzer QC included)
• qPCR (triplicate) -- using either Taqman or SybrGreen Chemistry
• Custom Assay -- Taqman qPCR optimization
• Custom Assay -- SybrGreen qPCR optimization
25
Inquire
29
10
Inquire
Inquire
31
Inquire
40
15
Inquire
Inquire
38
Inquire
44
18
Inquire
Inquire
Pyrosequencing/PyroMark (top)
On Campus
Non-Profit
Commercial
New Project Consultation
PCR and Pyrosequencing Run/Sample (one target region)
Run Only
• Self Service: Instrument run
-- Client prepares samples ready to run
Inquire
14
7
Inquire
Inquire
17
11
Inquire
Inquire
27
13
Inquire
Next Generation Sequencing (top)
On Campus
Non-Profit
Commercial
Sample Prep Full Service
• MiSeq Services:
MiSeq Run Only:
--
Client provides MiSeq reagent kit with samples preloaded into the cartridge.
• 2 x 25 cycles
• 2 x 75 cycles
• 2 x 150 cycles
• 2 x 250 cycles
• 2 x 300 cycles
MiSeq Set-up and Run:
-- PAN provides MiSeq reagent kit and initial sample QC on Agilent 2100 Bioanalyzer.
• 2 x 25 cycles
• 2 x 75 cycles
• 2 x 150 cycles
• 2 x 250 cycles
• 2 x 300 cycles
Inquire


180
291
365
515
625


878
1184
1258
1505
1615
Inquire


231
373
467
659
800


929
1266
1360
1649
1790
Inquire


335
541
679
958
1162


1033
1434
1572
1948
2152
DNA Sequencing & Fragment Analysis (top)
On Campus
Non-Profit
Commercial
• DNA Sequencing:
Per Sample Price:
Full Service
Intermediate Service (Clean-up & Run)
Economy Service (Run Only)
Per Plate Price:
• Full Service
• Run Only
• Fragment Analysis (GeneScan):
Clean-up and Run
Run only

6
4
3

490
170

6
4

9
6
5

795
325

9
7

11
7
6

950
490

11
10.50
Peptide Synthesis (top)
(Non-purified peptide synthesis includes analytical HPLC and Mass spec QC)
On Campus
Non-Profit
Commercial
• Stadard scale: (0.2 mmole-200 mg-500 mg)/amino acid (non purified)
• Set-up fee (includes first 5 Amino Acids)
• Per additional Amino Acid (>5 AA)
• Immunogen scale: (25 µmole-10 mg-30 mg)/amino acid (non-purified)
• Set-up fee (includes first 5 Amino Acids)
• Per additional Amino Acid (>5 AA)
• HPLC Purification
• Modification
**Shipping and Handling :
• USA
• International
220
48
126
26
220
Inquire
no charge
47

261
52

164
35
254
Inquire
26
47

340
74

239
51
360
Inquire
26
47
Mass Spectrometry (top)
On Campus
Non-Profit
Commercial
• Mass spectrometry by MALDI: (per sample)
• Peptides (500-5000Da)
• Other (including proteins, DNA, polymer, small molecules)
• Prespotted plate
• Zip Tip Cleanup

16
27
11
12

21
36
13
12

28
48
20
15
Protein Analytics (top)
On Campus
Non-Profit
Commercial
Mass Mapping: (Protein Identification)
-- In-gel digestion with trypsin; MS/MS peptide fragmentation; Mascot search against the NCBInr protein database; Report summary in pdf format.
• Price per sample (for first 10 samples in an order)
• Additional sample(s) (above 10 samples in the order)
• Specialized database construction
• Protein ID/Characterization from complex protein mixtures
Edman Sequencing:
• Set-up fee/sample (includes first 7 Residues)
• Per residue (>7 residues)

155
100
Inquire
Inquire

225
25

202
132
Inquire
N/A

258
36

280
187
Inquire
N/A

372
50
Surface Plasmon Resonance -- Biacore (top)
(Experimental set-up includes generation of biosensor surfaces for ligand capturing: Direct or indirect capture)
On Campus
Non-Profit
Commercial
• Set-up fee (per chip, user provides chip)
• Experiment:
• Full-day duration
• Half-day duration
• User-operated instrument session (per hour)
• Biacore 3000
• T200
• User-operated instrument session (per day)
• Biacore 3000
• T200
• Consulting or data analysis (half-day duration)
210
360
180

53
66

299
357
190
320

550
275

81
108

367
431
278
450

735
368

107
137

531
646
366
Antibody (top)
On Campus
Non-Profit
Commercial
› All fees
Inquire
Inquire
Inquire


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