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Service Information and Rates

Oligo Synthesis (Shipping and Handling) Peptide Synthesis (Shipping and Handling)
Gene Expression & Bioanalyzer QC Mass Spectrometry
Real-Time PCR Protein Analytics
   • Mass Mapping
   • Edman Sequencing
Pyrosequencing
Next Generation Sequencing Surface Plasmon Resonance (Biacore)
DNA Sequencing & Fragment Analysis Antibody
Fluidigm C1 Single Cell Services  

** We only accept PTA or PO# as a form of payment. We are currently not set up for any credit card transactions **


GENOMIC SERVICES
Oligo Synthesis (top)
On Campus
Non-Profit
Commercial
• DNA (per base):
• 40nmole (standard scale)
• 200nmole
• 1µmole
• DNA bulk orders (per base):
• 40nmole (8-15 oligos/order)
• 40nmole (16-23 oligos/order)
• 40nmole or 25nmole (>24 oligos/order)
• 25nmole - 96-well plate format by request (>48 oligos/order)
• RNA (per base):
• 40nmole
• 200nmole
• Purification (per oligo):
• 40nmole and 200nmole synthesis scales
• 1umole scale
• Modification:
• Biotin -- (**For any orders with 3 or more modifications/order, please inquire.)
• TagMan Probe reporters and quenchers
• Inosine
• dU
• 5' Phosphorylation
• 5' Thiolation
• Internal mixed bases
• Phosphothiolation (antisense)
• Other modifications
• LNA (per base)
**Shipping and Handling :
• USA
• International
0.39
0.99
2.10

0.32
0.27
0.12
0.12
7.20
9.20
9.50
19.10

50
60
10
10

10

Inquire
no charge
no charge
Inquire
Inquire
no charge
48
0.50
1.29
2.73

0.42
0.35
0.16
0.16
9.36
11.96
12.35
24.83

65
78
13
13
13
Inquire
no charge
no charge
Inquire
Inquire
27
48
0.70
1.79
3.78

0.58
0.49
0.21
0.21
12.96
16.56
17.10
34.38

90
108
18
18
18
Inquire
no charge
no charge
Inquire
Inquire
27
48
Gene Expression & Bioanalyzer (top)
On Campus
Non-Profit
Commercial
Expression Analysis: Full Services (** Chip is not included **)  
3' Array:
-- cDNA synthesis to data acquisition; includes Bioanalyzer QC
.
Standard (>50ng): using Affymetrix 3' IVT Express protocol.
Small (500pg - 50ng): using NuGen protocol or PAN 2-cycle amplification protocol.
Gene Array:
-- Whole Transcript (WT) Sense Target Labeling to data acquisition.
Standard (>50ng): using Affymetrix WT protocol.
Small (500pg - 2ng): using Affymetrix WT Pico protocol.
Human Transcriptome Array 2.0 (HTA 2.0)/Mouse Transcriptome Array 1.0 (MTA 1.0)/Rat Transcriptome Array 1.0 (RTA 1.0)
-- Interrogates exons for coding transcripts, exon-exon splice junctions and non-coding transcripts.
miRNA Array:
-- miRNA coverage of 71 organisms, including human, mouse, rat, canine, and rhesus macaque
.


440
485
425
430

490
200


572
630
552
559

637
260


792
873
765
774
882
360
Whole Genome DNA Analysis: Full Service (** Chip is not included **)  
-- Starting Material: genomic DNA
• Human SNP Array 5.0 (1 chip)
• Human SNP Array 6.0 (1 chip)
• Cytoscan

420
454
Inquire

546
590
Inquire

756
817
Inquire
Other Services  
Hybridization and Scanning (** Chip is not included **)
Agilent Bioanalyzer QC (per sample)
• Nano RNA or DNA 1000 (up to 12 samples), or Pico RNA (up to 11 samples)
         ° each additional sample
• Small RNA or High-Sensitivity DNA (up to 11 samples)
         ° each additional sample
93

15.50
12.50
19
16.50
121

21
17
25
22
167

28
23
35
30
Real-Time PCR (top)
On Campus
Non-Profit
Commercial
• Instrument run: (per plate)
-- Client prepares plate ready to run
• Full Service: (per sample)
• Set-up fee (per order) -- Prices do not include primers
• Reverse Transcription -- (Bioanalyzer QC included)
• qPCR (triplicate) -- using either Taqman or SybrGreen Chemistry
• Custom Assay -- Taqman qPCR optimization
• Custom Assay -- SybrGreen qPCR optimization
26
Inquire
30.50
10.50
Inquire
Inquire
34
Inquire
40
14
Inquire
Inquire
47
Inquire
55
19
Inquire
Inquire
Pyrosequencing/PyroMark (top)
On Campus
Non-Profit
Commercial
New Project Consultation
PCR and Pyrosequencing Run/Sample (one target region)
Run Only
• Self Service: Instrument run
-- Client prepares samples ready to run
Inquire
15
7.50
Inquire
Inquire
20
10
Inquire
Inquire
27
14
Inquire
Next Generation Sequencing (top)
On Campus
Non-Profit
Commercial
Sample Prep Full Service
• MiSeq Services:
MiSeq Run Only:
--
Client provides MiSeq reagent kit with samples preloaded into the cartridge.
• 2 x 25 cycles
• 2 x 75 cycles
• 2 x 150 cycles
• 2 x 250 cycles
• 2 x 300 cycles
MiSeq Set-up and Run:
-- PAN provides MiSeq reagent kit and initial sample QC on Agilent 2100 Bioanalyzer.
• 2 x 25 cycles
• 2 x 75 cycles
• 2 x 150 cycles
• 2 x 250 cycles
• 2 x 300 cycles
Inquire


180
291
365
515
625


878
1184
1258
1505
1615
Inquire


231
373
467
659
800


929
1266
1360
1649
1790
Inquire


335
541
679
958
1162


1033
1434
1572
1948
2152
DNA Sequencing & Fragment Analysis (top)
On Campus
Non-Profit
Commercial
• DNA Sequencing:
Per Sample Price:
Full Service
Intermediate Service (Clean-up & Run)
Economy Service (Run Only)
Per Plate Price:
• Full Service
• Run Only
• Fragment Analysis (GeneScan):
Clean-up and Run
Run only

7
5
4

550
195

7
5

9.10
7
6

715
254

9.10
7

12.60
9
7

950
351

13
9
Fluidigm C1 Single Cell Services (top)
On Campus
Non-Profit
Commercial
Fluidigm C1 Single Cell -- Instrument Run Only
-- Client provides Chip, C1 Single Cell Auto Prep Reagents, SMARTer Ultra Low RNA reagents and sample QC
Fluidigm C1 Single Cell Capture and cDNA Amplification (96 cell chip) -- Full Service
-- PAN provides C1 Single Cell Auto Prep Reagents, SMARTer Ultra Low RNA reagents and sample QC
NexteraXT cDNA Library Prep for Fluidigm (per sample)
• Fluidigm C1 Single Cell Capture and cDNA Amplification (800 cell chip) -- Full Service
-- PAN provides C1 Single Cell Auto Prep Reagents, SMARTer Ultra Low RNA reagents and sample QC
200
975
40
Inquire
260
1268
52
Inquire
360
1755
72
Inquire
PROTEIN SERVICES
Peptide Synthesis (top)
(Non-purified peptide synthesis includes analytical HPLC and Mass spec QC)
On Campus
Non-Profit
Commercial
• Stadard scale: (0.2 mmole-200 mg-500 mg)/amino acid (non purified)
• Set-up fee (includes first 5 Amino Acids)
• Per additional Amino Acid (>5 AA)
• HPLC Purification
• Modification
**Shipping and Handling :
• USA
• International
228
50
228
Inquire
no charge
48

295
65
295
Inquire
27
48

410
90
410
Inquire
27
48
Mass Spectrometry (top)
On Campus
Non-Profit
Commercial
• Mass spectrometry by MALDI: (per sample)
• Peptides (500-5000Da)
• Other (including proteins, DNA, polymer, small molecules)
• Prespotted plate
• Zip Tip Cleanup

17
29
12
13

22
38
15
17

31
52
22
23
Protein Analytics (top)
On Campus
Non-Profit
Commercial
Mass Mapping: (Protein Identification)
-- In-gel digestion with trypsin; MS/MS peptide fragmentation; Mascot search against the NCBInr protein database; Report summary in pdf format.
• Price per sample (for first 10 samples in an order)
• Additional sample(s) (above 10 samples in the order)
• Specialized database construction
• Protein ID/Characterization from complex protein mixtures
Edman Sequencing:
• Set-up fee/sample (includes first 7 Residues)
• Per residue (>7 residues)

155
100
Inquire
Inquire

265
29

202
132
Inquire
N/A

340
38

280
187
Inquire
N/A

470
53
Surface Plasmon Resonance -- Biacore (top)
(Experimental set-up includes generation of biosensor surfaces for ligand capturing: Direct or indirect capture)
On Campus
Non-Profit
Commercial
• Set-up fee (per chip, user provides chip)
• Experiment:
• Full-day duration
• Half-day duration
• User-operated instrument session (per hour)
• Biacore 3000
• T200
• User-operated instrument session (per day)
• Biacore 3000
• T200
• Consulting or data analysis (half-day duration)
225
386
193

57
70

320
382
203
315

542
271

85
115

420
495
285
460

750
375

115
147

580
690
390
Antibody (top)
On Campus
Non-Profit
Commercial
› All fees
Inquire
Inquire
Inquire


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