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Service Information and Rates

Oligo Synthesis (Shipping and Handling) Peptide Synthesis (Shipping and Handling)
Gene Expression & Bioanalyzer QC Mass Spectrometry
Real-Time PCR Protein Analytics
   • Mass Mapping
   • Edman Sequencing
Pyrosequencing
Next Generation Sequencing Surface Plasmon Resonance (Biacore)
DNA Sequencing & Fragment Analysis Antibody
Single Cell Genomics  

** We only accept PTA or PO# as a form of payment. We are currently not set up for any credit card transactions **
**Non-Profit and Commercial charges include administrative costs


GENOMIC SERVICES
Oligo Synthesis (top)
On Campus
Non-Profit
Commercial
• DNA (per base):
• 40nmole (standard scale)
• 200nmole
• 1µmole
• DNA bulk orders (per base):
• 40nmole (8-15 oligos/order)
• 40nmole (16-23 oligos/order)
• 40nmole or 25nmole (>24 oligos/order)
• 25nmole - 96-well plate format by request (>48 oligos/order)
• RNA (per base):
• 40nmole
• 200nmole
• Purification (per oligo):
• 40nmole and 200nmole synthesis scales
• 1umole scale
• Modification:
• Biotin -- (**For any orders with 3 or more modifications/order, please inquire.)
• TagMan Probe reporters and quenchers
• Inosine
• dU
• 5' Phosphorylation
• 5' Thiolation
• Internal mixed bases
• Phosphothiolation (antisense)
• Other modifications
• LNA (per base)
**Shipping and Handling :
• USA
• International
0.39
0.99
2.10

0.32
0.27
0.12
0.12
7.20
9.20
9.50
19.10

50
60
10
10

10

Inquire
no charge
no charge
Inquire
Inquire
no charge
49
0.50
1.29
2.73

0.42
0.35
0.16
0.16
9.36
11.96
12.35
24.83

65
78
13
13
13
Inquire
no charge
no charge
Inquire
Inquire
28
49
0.70
1.79
3.78

0.58
0.49
0.21
0.21
12.96
16.56
17.10
34.38

90
108
18
18
18
Inquire
no charge
no charge
Inquire
Inquire
28
49
Gene Expression & Bioanalyzer (top)
On Campus
Non-Profit
Commercial
Expression Analysis: Full Services (** Chip is not included **)  
3' Array:
-- cDNA synthesis to data acquisition; includes Bioanalyzer QC
.
Standard (>50ng): using Affymetrix 3' IVT Plus protocol.
Small (500pg - 50ng): using Affymetrix 3' IVT Pico protocol
Gene Array:
-- Whole Transcript (WT) Sense Target Labeling to data acquisition.
Standard (>50ng): using Affymetrix WT Plus protocol.
Small (500pg - 2ng): using Affymetrix WT Pico protocol.
Human Transcriptome Array 2.0 (HTA 2.0)/Mouse Transcriptome Array 1.0 (MTA 1.0)/Rat Transcriptome Array 1.0 (RTA 1.0)
-- Interrogates exons for coding transcripts, exon-exon splice junctions and non-coding transcripts.
miRNA Array:
-- miRNA coverage of 71 organisms, including human, mouse, rat, canine, and rhesus macaque
.


450
485
435
440

501
200


585
630
565
572

651
260


810
873
782
792
902
360
Whole Genome DNA Analysis: Full Service (** Chip is not included **)  
-- Starting Material: genomic DNA
• Human SNP Array 5.0 (1 chip)
• Human SNP Array 6.0 (1 chip)
• Cytoscan

420
454
Inquire

546
590
Inquire

756
817
Inquire
Other Services  
Hybridization and Scanning (** Chip is not included **)
Agilent Bioanalyzer QC (per sample)
• Nano RNA or DNA 1000 (up to 12 samples), or Pico RNA (up to 11 samples)
         ° each additional sample
• Small RNA or High-Sensitivity DNA (up to 11 samples)
         ° each additional sample
98

16
13
20
17
127

22
18
26
23
175

29
24
37
32
Real-Time PCR (top)
On Campus
Non-Profit
Commercial
• Instrument run: (per plate)
-- Client prepares plate ready to run
• Full Service: (per sample)
• Set-up fee (per order) -- Prices do not include primers
• Reverse Transcription -- (Bioanalyzer QC included)
• qPCR (triplicate) -- using either Taqman or SybrGreen Chemistry
• Custom Assay -- Taqman qPCR optimization
• Custom Assay -- SybrGreen qPCR optimization
27
Inquire
33
12
Inquire
Inquire
35
Inquire
43
16
Inquire
Inquire
48
Inquire
59
21.50
Inquire
Inquire
Pyrosequencing/PyroMark (top)
On Campus
Non-Profit
Commercial
New Project Consultation
PCR and Pyrosequencing Run/Sample (one target region)
• Run Only
-- PAN captures biotinylated product and runs client generated PCR products

Self Service: Instrument Run Only
-- Client prepares and runs plate; Reagents and consumables are not included but can be purchased from PAN.

Inquire
16
8
27
Inquire
21
11
35
Inquire
28
15
48
Next Generation Sequencing (top)
On Campus
Non-Profit
Commercial
Sample Prep Full Service
• MiSeq Services:
MiSeq Run Only:
--
Client provides MiSeq reagent kit with samples preloaded into the cartridge.
• 2 x 25 cycles
• 2 x 75 cycles
• 2 x 150 cycles
• 2 x 250 cycles
• 2 x 300 cycles
MiSeq Set-up and Run:
-- PAN provides MiSeq reagent kit and initial sample QC on Agilent 2100 Bioanalyzer.
• 2 x 25 cycles
• 2 x 75 cycles
• 2 x 150 cycles
• 2 x 250 cycles
• 2 x 300 cycles
Inquire


180
291
365
515
625


878
1184
1258
1505
1615
Inquire


231
373
467
659
800


929
1266
1360
1649
1790
Inquire


335
541
679
958
1162


1033
1434
1572
1948
2152
DNA Sequencing & Fragment Analysis (top)
On Campus
Non-Profit
Commercial
• DNA Sequencing:
Per Sample Price:
Full Service
Intermediate Service (Clean-up & Run)
Economy Service (Run Only)
Per Plate Price:
• Full Service
• Run Only
• Fragment Analysis (GeneScan):
Clean-up and Run
Run only

7.25
5.25
4.15

565
199

7.25
5.25

9.40
7.25
6.20

735
260

9.40
7.25

13
9.50
7.25

950
361

13.40
9.30
Single Cell Genomics (top)
On Campus
Non-Profit
Commercial
Single Cell -- Instrument Run Only (Fluidigm C1)
-- Client provides Chip, C1 Single Cell Auto Prep Reagents, SMARTer Ultra Low RNA reagents and sample QC
Single Cell Capture and cDNA Amplification (96 cell chip) -- Full Service (** Chip is not included **)
-- PAN provides Single Cell Auto Prep Reagents, SMARTer Ultra Low RNA reagents and sample QC
NexteraXT cDNA Library Prep (per sample)
Single Cell Capture and cDNA Amplification (800 cell chip) -- Full Service (** Chip is not included **)
-- PAN provides Single Cell Auto Prep Reagents, SMARTer Ultra Low RNA reagents and sample QC
200
975
41
Inquire
260
1268
53
Inquire
360
1755
74
Inquire
PROTEIN SERVICES
Peptide Synthesis (top)
(Non-purified peptide synthesis includes analytical HPLC and Mass spec QC)
On Campus
Non-Profit
Commercial
• Stadard scale: (0.2 mmole-200 mg-500 mg)/amino acid (non purified)
• Set-up fee (includes first 5 Amino Acids)
• Per additional Amino Acid (>5 AA)
• HPLC Purification
• Modification
**Shipping and Handling :
• USA
• International
235
51
233
Inquire
no charge
49

304
66
300
Inquire
28
49

422
92
418
Inquire
28
49
Mass Spectrometry (top)
On Campus
Non-Profit
Commercial
• Mass spectrometry by MALDI: (per sample)
• Peptides (500-5000Da)
• Other (including proteins, DNA, polymer, small molecules)
• Prespotted plate
• Zip Tip Cleanup

17.50
30
12
13.50

23
39
15
18

32
53
22
24
Protein Analytics (top)
On Campus
Non-Profit
Commercial
Mass Mapping: (Protein Identification)
-- In-gel digestion with trypsin; MS/MS peptide fragmentation; Mascot search against the NCBInr protein database; Report summary in pdf format.
• Price per sample (for first 10 samples in an order)
• Additional sample(s) (above 10 samples in the order)
• Specialized database construction
• Protein ID/Characterization from complex protein mixtures
Edman Sequencing:
• Set-up fee/sample (includes first 7 Residues)
• Per residue (>7 residues)

159
103
Inquire
Inquire

265
29

208
136
Inquire
N/A

340
38

288
193
Inquire
N/A

470
53
Surface Plasmon Resonance -- Biacore (top)
(Experimental set-up includes generation of biosensor surfaces for ligand capturing: Direct or indirect capture)
On Campus
Non-Profit
Commercial
• Set-up fee (per chip, user provides chip)
• Experiment:
• Full-day duration
• Half-day duration
• User-operated instrument session (per hour)
• Biacore 3000
• T200
• User-operated instrument session (per day)
• Biacore 3000
• T200
• Consulting or data analysis (half-day duration)
240
425
206

61
75

341
407
217
335

596
289

91
123

448
528
304
490

825
400

123
157

619
736
416
Antibody (top)
On Campus
Non-Profit
Commercial
› All fees
Inquire
Inquire
Inquire


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