- Turn-around time? (top)
One week for samples submitted by members of the Stanford community; two weeks or longer for off campus clients.
- In what form should samples be submitted? (top)
Best is adsorbed to PVDF membrane, usually following blotting from an SDS-PAGE gel. Only PVDF can be used. Nitrocellulose, for example, is absolutely forbidden. Alternatively, protein/peptides can be submitted as a dry powder, such as synthetic peptides, or in solution. Unless adsorbed to PVDF membrane, you must provide us with a complete list of ALL components of your sample (buffers, solvents, solutes, etc.) before we will undertake it’s analysis.
- How much sample do you need for the analysis? (top)
Blotted samples should contain 100 pmol of protein. Proteins or peptides dried or in solution may be identified at a level as low as 5 pmol.