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Located in rooms
B065 and B017
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RNA/DNA QC - Agilent Bioanalyzer QC
 

SUBMISSION REQUIREMENTS

 

• Sample Concentration Requirements --
    Please use the following table as a guide to get the right concentration for your samples for the assay requested.

Starting Material Sample Type Assay Type

Size Analysis Range

* Sample Concentration
- Detection Range

DNA
dsDNA, cDNA, genomic DNA, NGS DNA
• DNA 1000
• High-Sensitivity DNA
25 - 1000 bp
50 - 7000 bp
1ng/ul-50ng/ul
5pg/ul-5ng/ul
RNA
Total RNA, mRNA, cRNA, fragmented RNA, microRNA
• Nano RNA (Eukaryote, Plant, Prokaryote)
• Pico RNA (Eukaryote, Plant, Prokaryote)
• Small RNA (6-150 nt)
25 - 4000 nt
25 - 4000 nt
6 - 150 nt
5ng/ul-500ng/ul
50pg/ul-10ng/ul
50pg/ul-2ng/ul
* Although the Bioanalyzer is capable of detecting the lowest concentration amounts shown, we would recommend to have your sample(s) in the higher detection range for better results.

• Sample Submission --
    Samples should be submitted and labeled as followed:

  • Volume: 3ul (for DNA/RNA samples) -- NOT your entire stock.
  • Labels (on tube, clearly): Customer initials_ last 3 digits of the Run number shown on the order summary.

** After placing your order online, please bring your samples along with a copy of the order summary to PAN, Beckman Center, Rm. B065. Place your order form in the bin on the white freezer door. The samples should be placed in the appropriate rack in the freezer, top shelf, for either DNA or RNA samples.