» Real-Time PCR
 

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Located in rooms
B065 and B017
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Real-Time PCR
 

FREQUENTLY ASKED QUESTIONS
  1. Where is the PAN Facility located/contact info?
  2. What type of real-time instrument do you have?
  3. How do I schedule a run (self-service) for the StepOnePlus?
  4. How much does it cost?
  5. What primer/assays does the PAN Facility provide?
  6. What concentration should the RNA/DNA/primers be?
  7. How much sample is needed for Full Service?
  8. Where can I get more information on real-time qPCR?



1. Where is the PAN Facility located/contact info? (top)
We are located in the basement of the Beckman Center, B017.  We also have a drop-off area in the cold room B024.

Phone: (650) 723-3189
Email: dnaseq@stanford.edu
Shipping Address:
 
PAN Facility - Real-Time qPCR
Beckman Center, B017
279 Campus Drive, West
Stanford, Ca 94305


2. What type of real-time instrument do you have? (top)
We have an ABI/Life Technologies StepOnePlus Real-Time PCR instrument. For more information on the instrument, including manuals and free software please visit their website http://www.lifetechnologies.com/order/catalog/product/4376600


3. How do I schedule a run (self-service) for the StepOnePlus? (top)
There are three steps you need to take in order to use the self-service option.
1) Create an account with the PAN Facility. This is for billing purposes.
2) Create an account with the StepOne Calendar.
3) Schedule a quick 15-30min tutorial with a member of the PAN Facility on how to use the StepOnePlus instrument

Please contact us if you're running late, we can usually wait.


4. How much does it cost? (top)
Please check our current price list.


5. What primers does the PAN Facility provide? (top)
Unfortunately, we do not provide any primers or assays for the real-time qPCR service. We can provide a copy of the Primer Express software and suggestions on how to design primers.


6 . What concentration should the DNA be? (top)

    Template
    Concentration (F.C.)
    260/280
    260/230
    RNA*
    10ng - 2ug
    2.0
    2.0-2.2
    cDNA**
    1ng/ul - 10ng/ul
    N/A
    N/A
    DNA*
    1ng/ul - 10ng/ul
    1.8
    2.0-2.2
    Primer/Assay
    300nM - 900nM
       

    *RNA/DNA must be measured on a Nanodrop and/or BioAnalyzer.
    **Assume 100% conversion of RNA to cDNA and dilute RT accordingly.

7. How much sample is needed for Full Service? (top)
Submission requirement for Full Service: at least 16ul/sample. Please contact us if you don't have enough sample for submission.


8. Where can I get more information on real-time qPCR? (top)
Helpful Links :
http://www.lifetechnologies.com/us/en/home/life-science/pcr/real-time-pcr.html
http://www.eurogentec.com/EGT/files/qPCR-guide.pdf
http://www.qiagen.com/knowledge-and-support/resource-center/resource-download.aspx?id=f7efb4f4-fbcf-4b25-9315-c4702414e8d6&lang=en