» Real-Time PCR

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  Genomic Services
• Oligo Synthesis
• Gene Expression
• Real-Time PCR
• Pyrosequencing
• Next Gen Sequencing
• DNA Sequencing
   & Fragment Analysis
• Single Cell Genomics
  Protein Services
• Peptide Synthesis
• Mass Spectrometry
• Protein Analytics
‡ Mass Mapping
‡ Edman Sequencing
• SPR (Biacore)
Located in rooms
B065 and B017


Real-Time PCR

  • Currently the instrument is available for Self-Service with the researcher providing all StepOnePlus compatable consumables and reagents. Please follow the instructions below to for reservation and usage information:

    • Create two accounts.
      § (1) with the PAN Facility for billing purposes. You only need to do this once. Update PTA/PO as necessary.
      § (2) with StepOnePlus Calendar for scheduling run(s).

    • First-time users must contact us for a free and quick tutorial on instrument and software before scheduling a run.

    • StepOnePlus compatible consumables and reagents only. Read consumable/reagent details for this information.
      § ABI MicroAmp Fast Optical Plate (0.1ml) - Part #4346907 (available at PAN for $4.00/plate).
      § ABI MicroAmp Optical Adhesive Film - Part #4360954 (available at PAN for $2.00/cover).

    • Free copy of the StepOnePlus software (PC only) is available through ABI/Life Technologies or through PAN.

    • Bring plate wrapped in foil. Do not place plate on ice, as ice may generate false flurescent signal. Plate is okay at RT for a short time. If plate needs to be storaged for a few hours or a day you may store it in our fridge.

    • Bring plate layout and thermal cycling conditions for your SybrGreen or Taqman reagent with you.

    • A centrifuge is available in the lab for customer use.

    • After hour access is available, please contact panpyro-qpcr@stanford.edu or Mike Eckart for door codes.

    **Please note that the charge is per plate, not per hour, refer to the price sheet for detailed information.