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  Genomic Services
• Oligo Synthesis
• Gene Expression
• Real-Time PCR
• Pyrosequencing
• Next Gen Sequencing
• DNA Sequencing
   & Fragment Analysis
• Single Cell Genomics
• RNA/DNA QC
‡ Bioanalyzer QC
‡ Fragment Analyzer QC
  Protein Services
• Peptide Synthesis
• Mass Spectrometry
• Protein Analytics
‡ Mass Mapping
‡ Edman Sequencing
• Biacore
• Antibody
 
 
Located in rooms
B065 and B017
bklogo

 

Single Cell Genomics/Fragment Analyzer QC
 

OVERVIEW

PAN is offering different services for Single Cell Genomics technology. You must check the requirements for each service before submitting your samples to us.

 

SERVICE OPTIONS:
Our service can be tailored to individual requirement. Please contact us to discuss your individual requirements.


Instrument Run Only : (back to top)

• General Info. --

- You can check the machine availabililty and make reservation from PAN Scheduler.
- Please make sure that you booked the right instrument since there are other resources listed on the same scheduler.
-
Upon the approval of PAN staff, you wiill be able to use the machine at the scheduled time.
- Please contact us if there's any delay in getting your reservation(s) approved.



Full Service: (back to top)

• General Info. --

Coming Soon...



Fragment Analyzer QC: (back to top)

General Info. --

The Advanced Analyztical Fragment Analyzer is a capillary electrophoresis based platform that provides sizing quant and qyality colntorl of DNA and RNA.  PAN Facility carries a 96 capillary instrument that can process one 96 well plate in approximately one hour. 

* Fragment resolution from 10bp to 40,000bp
* Resolution down to 3bp
* Detection from 5 pg/ul

 

§ Assays and their Applications --

     PAN Facility currently offers different assays for a wide range of applications:

  • DNA Assays

    * NGS Library QC

    * Large Fragment QC
    * Genomic DNA QC

  • RNA Assays:

    * Small RNA QC (5nt-200nt)

    * Standard Sensitivity RNA QC (15nt-6,000nt)
    * High Sensitivity RNA QC (15nt-6000nt)

Please use the following table as a guide to choose the right assay for your samples. Before submitting your samples, you must also check out the concentration requirements for the assay chosen since each assay has different sensitivity level.

Starting Material Kit Type Assay Type

Size Analysis Range

* Sample Concentration
- Detection Range

DNA
NGS DNA
• NGS Library QC (Standard Sensitivity)
• NGS Library QC (High Sensitivity)
• NGS Library QC (High Sensitivity Small Fragment)
25bp - 5,000bp
25bp - 5,000bp
50bp - 1,500bp
0.1ng/ul - 10ng/ul
5pg/u l- 500pg/ul
5pg/ul - 500pg/ul
Large Fragment
• Large Fragment QC (Standard Sensitivity)
• Large Fragment QC (High Sensitivity)
• Large Fragment 50kb QC (High Sensitivity)
50bp - 20,000bp
50bp - 20,000bp
75bp - 48,500bp
0.1ng/ul - 10ng/ul
5pg/ul - 500pg/ul
5pg/ul - 600pg/ul
gDNA
• gDNA QC (Standard Sensitivity)
• gDNA QC (High Sensitivity)
• gDNA 50kb QC
50bp - 20,000bp
50bp - 20,000bp
75bp - 48,500bp
5ng/ul - 200ng/ul
50pg/ul - 5ng/ul
25ng/ul - 250ng/ul
RNA
 
• Small RNA
• RNA (Standard Sensitivity)
• RNA (High Sensitivity)
5nt - 200nt
15nt - 6000nt
15nt - 6000nt
50pg/ul - 2ng/ul
25ng/ul - 500ng/ul
500pg/ul - 5ng/ul


• Data Release --

The data generated from the Advanced Analytical Fragment Analyzer instrument will be emailed to you in .pdf and .xls format.

  • PDF file - contains run summary, method information, gel image, and trace for each sample.
  • Excel file - contains concentration, molarity, and size information.

• Data Analysis --

Our data interpretation guide will help you understand your data better. If you still have questions/concerns regarding your data, please feel free to contact us at panbioanalyzer@stanford.edu.


• Turn-around Time --

Orders will be processed within 2 days of submission.



Nextera Library Prep: (back to top)

• General Info. --

Coming Soon...