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  Genomic Services
• Oligo Synthesis
• Gene Expression
• Real-Time PCR
• Pyrosequencing
• Next Gen Sequencing
• DNA Sequencing
   & Fragment Analysis
• Single Cell Genomics
• RNA/DNA QC
‡ Bioanalyzer QC
‡ Fragment Analyzer QC
  Protein Services
• Peptide Synthesis
• Mass Spectrometry
• Protein Analytics
‡ Mass Mapping
‡ Edman Sequencing
• Biacore
• Antibody
 
 
Located in rooms
B065 and B017
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Gene Expression
 

SUBMISSION REQUIREMENTS

** There are different types of service provided within the Gene Expression section. Please check for the appropriate sample submission requirements for each service before placing your orders.


Full Service: (back to top)

• Sample Amount Requirements --

Array Type

Starting Material

Assay Type

Amount required for *one* sample

3’ Array - Standard
3' Array - Small

Total RNA

3’IVT Express 50-500ng
500pg - 50ng
Gene Array

Total RNA

Ambion WT Expression followed by Affymetrix GeneChip WT Terminal Labeling kit 50-500ng
Exon Array

Total RNA

Ambion WT Expression followed by Affymetrix GeneChip WT Terminal Labeling kit 50-500ng
Tiling Array

Total RNA

Affymetrix GeneChip WT ds Target or ChIP 3-5ug
miRNA Array

miRNA

GeniSphere 3DNA FlashTag Biotin HSR 100-1000ng
HTA

Total RNA

Ambion WT Expression followed by Affymetrix GeneChip WT Terminal Labeling kit 50-500ng
Human SNP

ds DNA

Affymetrix Genome-Wide Human SNP Nsp/Sty 6.0 0.5-1ug or 50ng/ul

* The assay for 3’ Array will be chosen based on the available RNA quantity.


• Sample Submission --

  • Please log in to your account (or create a new one) to place your order.
  • Choose Gene Expression section.
  • Sample Target Labeling depends on the array. Choose the appropriate labeling and proceed with the rest of the order form.
    ** The minimum amount of total RNA required is 50ngs. We prefer to have between 100ng-500ng for each sample, if possible. If you have less than 50ng, please select Small Target Labeling.  This implies a two-cycle amplification protocol. Please contact the staff in the Gene Expression team for specific information about this protocol.
  • When done, please print out the form and bring it, together with your samples, to PAN. No appointment necessary.



Hybridization and Scanning : (back to top)

• Sample Concentration Requirements --

Array Type

Hybridization Target

Target Concentration

3’ Array Biotin-labeled, fragmented cRNA 49/64 array format: 15ug in 40ul*
100 array format (Midi): 12ug in 30ul
169 array format (Mini): 7.5ug in 20ul
Gene 1.0ST Array Biotin-labeled, fragmented ss DNA ~27ul**
Exon Array Biotin-labeled, fragmented ss DNA ~60ul**
Tiling Array Biotin-labeled, fragmented ds DNA ~7.5ug (~60ul)***
miRNA Array GeniSphere 3DNA FlashTag Biotin HSR 21ul
Human SNP 6.0 Array Biotin-labeled, fragmented ds DNA ~73ul

* If using Affymetrix 3’IVT Express protocol
** If using Affymetrix GeneChip WT Terminal Labeling protocol
***If using Affymetrix GeneChip WT Double-Stranded Target protocol



Bioanalyzer QC : (back to top)

• Sample Concentration Requirements --
    Please use the following table as a guide to get the right concentration for your samples for the assay requested.

Starting Material Sample Type Assay Type

Size Analysis Range

* Sample Concentration
- Detection Range

DNA
dsDNA, cDNA, genomic DNA, NGS DNA
• DNA 1000
• High-Sensitivity DNA
25 - 1000 bp
50 - 7000 bp
1ng/ul-50ng/ul
5pg/ul-5ng/ul
RNA
Total RNA, mRNA, cRNA, fragmented RNA, microRNA
• Nano RNA (Eukaryote, Plant, Prokaryote)
• Pico RNA (Eukaryote, Plant, Prokaryote)
• Small RNA (6-150 nt)
25 - 4000 nt
25 - 4000 nt
6 - 150 nt
5ng/ul-500ng/ul
50pg/ul-10ng/ul
50pg/ul-2ng/ul
* Although the Bioanalyzer is capable of detecting the lowest concentration amounts shown, we would recommend to have your sample(s) in the higher detection range for better results.

• Sample Submission --
    Samples should be submitted and labeled as followed:

  • Volume: 3ul (for DNA/RNA samples) -- NOT your entire stock.
  • Labels (on tube, clearly): Customer initials_ last 3 digits of the Run number shown on the order summary.

** After placing your order online, please bring your samples along with a copy of the order summary to PAN, Beckman Center, Rm. B065. Place your order form in the bin on the white freezer door. The samples should be placed in the appropriate rack in the freezer, top shelf, for either DNA or RNA samples.