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RNA-DNA QC
 

SUBMISSION REQUIREMENTS

** Please check for the appropriate sample submission requirements for each machine before placing your orders.


Agilent Bioanalyzer QC : (back to top)

• Sample Concentration Requirements --
    Please use the following table as a guide to get the right concentration for your samples for the assay requested.

Starting Material Sample Type Assay Type

Size Analysis Range

* Sample Concentration
- Detection Range

DNA
dsDNA, cDNA, genomic DNA, NGS DNA
• DNA 1000
• High-Sensitivity DNA
25 - 1000 bp
50 - 7000 bp
1ng/ul-50ng/ul
5pg/ul-5ng/ul
RNA
Total RNA, mRNA, cRNA, fragmented RNA, microRNA
• Nano RNA (Eukaryote, Plant, Prokaryote)
• Pico RNA (Eukaryote, Plant, Prokaryote)
• Small RNA (6-150 nt)
25 - 4000 nt
25 - 4000 nt
6 - 150 nt
5ng/ul-500ng/ul
50pg/ul-10ng/ul
50pg/ul-2ng/ul
* Although the Bioanalyzer is capable of detecting the lowest concentration amounts shown, we would recommend to have your sample(s) in the higher detection range for better results.

• Sample Submission --
    Samples should be submitted and labeled as followed:

  • Volume: 3ul (for DNA/RNA samples) -- NOT your entire stock.
  • Labels (on tube, clearly): Customer initials_ last 3 digits of the Run number shown on the order summary.

** After placing your order online, please bring your samples along with a copy of the order summary to PAN, Beckman Center, Rm. B065. Place your order form in the bin on the white freezer door. The samples should be placed in the appropriate rack in the freezer, top shelf, for either DNA or RNA samples.



AATI Fragment Analzyer QC : (back to top)

• Sample Concentration Requirements --
    Please use the following table as a guide to get the right concentration for your samples for the assay requested.

Starting Material Kit Type Assay Type

Size Analysis Range

* Sample Concentration
- Detection Range

DNA
NGS DNA
• NGS Library QC (Standard Sensitivity)
• NGS Library QC (High Sensitivity)
• NGS Library QC (High Sensitivity Small Fragment)
25bp - 5,000bp
25bp - 5,000bp
50bp - 1,500bp
0.1ng/ul - 10ng/ul
5pg/u l- 500pg/ul
5pg/ul - 500pg/ul
Large Fragment
• Large Fragment QC (Standard Sensitivity)
• Large Fragment QC (High Sensitivity)
• Large Fragment 50kb QC (High Sensitivity)
50bp - 20,000bp
50bp - 20,000bp
75bp - 48,500bp
0.1ng/ul - 10ng/ul
5pg/ul - 500pg/ul
5pg/ul - 600pg/ul
gDNA
• gDNA QC (Standard Sensitivity)
• gDNA QC (High Sensitivity)
• gDNA 50kb QC
50bp - 20,000bp
50bp - 20,000bp
75bp - 48,500bp
5ng/ul - 200ng/ul
50pg/ul - 5ng/ul
25ng/ul - 250ng/ul
RNA
 
• Small RNA
• RNA (Standard Sensitivity)
• RNA (High Sensitivity)
5nt - 200nt
15nt - 6000nt
15nt - 6000nt
50pg/ul - 2ng/ul
25ng/ul - 500ng/ul
500pg/ul - 5ng/ul

• Sample Submission --
    Samples should be submitted in plate format.

** After placing your order online, please bring your sample plates along with a copy of the order summary to PAN, Beckman Center, Rm. B065. Place your order form in the bin on the white freezer door.