PAN_SCG

PAN: Protein and Nucleic Acid Facility

» Single Cell Genomics

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Genomic Services

• Next Gen Sequencing

• DNA Sequencing
& Fragment Analysis

• Single Cell Genomics

Protein Services

Located in rooms
B065 and B017

• OVERVIEW

SERVICE OPTIONS:
Our service can be tailored to individual requirement. Please contact us to discuss your individual requirements.

- BD Rhapsody - Single cell analysis system

- Single Cell Library Prep

- Spatial Transcriptomics

* Vizgen Merscope

* Curio Seeker and Curio Trekker

* 10X Visium

BD Rhapsody HT Express System: (back to top)

• General Info. -- more details...

- The system enables single-cell capture and barcoding of hundreds to thousands of single cells for analysis of genomic and proteomic information, using a gentle androbust microwell-based single-cell partitioning technology.
- The captured cellular information is utilized to generate various types of libraries for next-generation sequencing applications providing accelerated time to insight.

Single Cell Library Prep: (back to top)

• General Info. --

** LIbrary prep will include sample QC

** Single Cell RNA Seq - Ultra low input RNA-Seq using Clontech SMART-Seq Kit

** scATAC-Seq

Spatial Transcriptomics: (back to top)

• Vizgen Merscope

- MERSCOPE is the complete platform solution for MERFISH technology, which provides massively multiplexed, error-robust, single-cell in situ transcriptomic imaging.
- Multiplexed error-robust fluorescence in situ hybridization (MERFISH) is a spatially resolved
single-cell transcriptome profiling technology. It enables researchers to map the molecular, cellular, and functional composition of biological systems with spatial context.

• Curio Seeker and Curio Trekker

Curio Seeker Spatial Transcriptomics Kit:
- Enable whole transcriptome, spatial mapping of fresh frozen tissues at industry-leading resolution.

Curio Trekker:
- Single-cell mapping kit, which adds valuable spatial information to single-nucleus transcriptomics data without compromising on cellular resolution or transcriptome coverage.
- It works by tagging each nucleus within its native tissue environment with unique spatial barcodes. These spatial barcodes are read using next-generation sequencing (NGS), allowing each nucleus to be bioinformatically positioned in its spatial coordinates. The result is a spatial map with true single-cell resolution without the use of complex instrumentation, cell-type deconvolution, or cell segmentation.

• 10X Visium

* General Info. -- Coming soon...