Attention: This service is temporarily discontinued. Please contact Mike Eckart if you have any questions or concerns. Thanks.
The PAN Biotechnology Facility offers several services for identifying proteins and for determining primary sequence information for proteins and peptides.
• Mass Mapping is a convenient and sensitive method for determining the identity of proteins. Mass mapping involves tryptic digestion of proteins and mass analysis of the resulting mixture of peptides. As long as a protein’s sequence is present in a database, it can be accurately identified by this method. Samples can be submitted in gels (preferred), in solution, or dried. Mass Mapping is performed using the 4700 Proteomics Analyzer.
• Edman Sequencing provides primary sequence information by automated analysis of sequential amino acid residues. Samples can be submitted immobilized on PVDF membranes (preferred), in solution or dried samples. For Stanford clients, we can extend the method to include protease digestion, separation by HPLC and subsequent Edman sequencing of peptides.